Ten strains of mesophilic sulfatereducing bacteria srb were isolated from subgingival plaque in periodontal lesions of ten patients with periodontitis. Sulfatereducing bacteria srb comprise a large number of phylogenetically diverse prokaryotes that have in common their ability to grow by catalysing the dissimilatory reduction of sulfate to sulphide in anoxic environments muyzer and stams 2008. Sulfatereducing bacteria srb are a widespread group of microorganisms that are often isolated from the anoxygenic environments lake depths, soil, or swamps, and they are also present in the human and animal intestines. The isolation and cultivation of sulphate reducing bacteria.
Corrosionpedia what are sulfatereducing bacteria srb. They may be isolated by using a variety of media over a wide range of temperature. The isolation and cultivation of sulphatereducing bacteria. Sulfatereducing bacteria were enumerated in soils and water samples from. Isolation of chemotactic sulfate reducing bacteria. Three strains were identified as members of the genera desulfotomaculum and desulfosporosinus. When rice plants were severely injured by sulfides, maximum densities ranged from 10 7 to 10 9 bacteria g. Isolation of sulfatereducing bacteria from deep sediment layers of the paci. The tubes were brimfilled with medium and closed to provide anaerobic conditions. Pdf isolation and characterization of aggregateforming. Sulfate reducing bacterium an overview sciencedirect. Sridar2 1paddy processing research centre, thanjavur4, tamil nadu, india.
In this process, sulfate reduction permits the dissimilatory oxidation of organic matter with the release of hydrogen sulfide, a corrosive and cytotoxic compound. Isolation and characterization of bacteria isolated from municipal solid waste for production of industrial enzymes and waste degradation. Formate, fatty acids c4 to c16 and hydrogen were readily. Hungate roll tube technique is generally used for anaerobic isolation, wherein roll tubes are employed for isolation of bacterial colonies. Physiological and biochemical characterization was done by bergeys manual of determinative bacteriology.
Keywords sulfate reducing bacteria srb, ironlyngby medium, barite mine, barium sulphate, enterobacter species and bacillus species the present study was aimed at isolating sulfate reducing bacteriasrb by enriched method using ironlyngby medium from barium sulphate mines situated in mangampeta, kadapa district of andhra pradesh. Merck provides high quality media for the enrichment, isolation and enumeration of clostridia sulfite reducing anaerobic bacteria. Through a synthesis of research and case studies of srb treatment at coal and hardrock mine sites, it will present. Isolation and identification of sulfate reducing bacterial strains indigenous to sulphur rich barite mines g.
Intestinal sulfatereducing bacteria are often isolated from patients with inflammatory bowel disease, including ulcerative colitis, and can be involved in the development of gut inflammation. Sulfatereducing bacteria are a complex physiologic group and classifying them has traditionally required the consideration of several properties, the most important of which are motility, cell shape, the guanine plus cytosine content of dna, the presence of desulfoviridin and cytochromes, growth temperature, use of various electron donors, and. Quantitative analysis of three hydrogenotrophic microbial. Isolation and identification of sulfate reducing bacteria. Isolation of sulfur reducing and oxidizing bacteria found.
Pdf on a global scale, crustal fluids fuel a substantial part of the deep subseafloor biosphere by providing electron acceptors for microbial. Isolation of a nitratereducing bacteria strain from oil. These consistent culture media are available in a variety of formulations as dehydrated culture media in the form of granules. Isolation of a nitratereducing bacteria strain from oil field brine and the inhibition of sulfatereducing bacteria zhuang wen 1,3,5, duan jizhou 4, chu liye 2 and shao hongbo 1,2 1the casshandong provincial key laboratory of coastal environmental process, yantai institute of costal. These bacteria appear to cause corrosion by producing a highly corrosive product in. Monitoring enrichment andisolation of sulfatereducing. Isolation and molecular characterization of sulfate. Isolation of sulfatereducing bacteria from tunisian marine sediments and description of desulfovibrio bizertensis sp. It was noted that an increase in iron and sulfate reducing bacteria were more pronounced closer to the landfill. Cultivation and detection of sulfate reducing bacteria. In the metabolic activities, the srb can reduce sulfate to h2s which immediately binds to metals that are widely available on mined. Isolation and characterization of bacteria isolated from. Isolation and identification of sulfate reducing bacteria srb from.
Isolation of sulfatereducing bacteria from human thoracoabdominal pus julien loubinoux,1,2 benoit jaulhac, 3yves piemont, henri monteil,3 and alain e. One of these strains, designated mb3t, was characterized further. Pdf isolation of sulfatereducing bacteria from sediments above. Overall, most studies conclude that the iron and sulfatereducing species exist beneath the landfill. Paramageetham2 1department of botany, sri venkateswara university, tirupati517502, india 2department of microbiology, sri venkateswara university, tirupati517502, india. Although some acidophilic and alkaliphilic species have been described recently, most of the known sulfate. There are many strains and perhaps several species of sulphate reducing bacteria. The species of sulfatereducing bacteria that prevail in sites affected by periodontal disease may be different from those commonly occurring in the digestive tracts of healthy individuals. Seven nonsporulating, mesophilic strains were isolated.
Dna isolation isolation and purification of dna was carried out with three day old culture of sulfatereducing bacteria using a qiamp dna mini kit qiagen, cat. Pdf isolation and purification of sulfatereducing bacteria. Title isolation and identification of sulfate reducing bacteria srb of sediment pond former coal mine in samarinda, east kalimantan. Pdf isolation and identification of sulfate reducing bacterial strains.
The present study was aimed at isolating sulfate reducing bacteriasrb by. Sulfatereducing bacteria srb are bacteria that can obtain energy by oxidizing organic compounds or molecular hydrogen while reducing sulfate to hydrogen sulfide. Therefore, these sulfidogenic microorganisms breathe sulfate rather than. Sulfate reducing bacteria srb are a group of genetically similar anaerobic organisms that were first discovered by hamilton 1994. Sulfate reducers were always obtain as well as acetogenes. The method used in this isolation is pour plate method in the medium desulfovibrio medium with lactate under anaerobic conditions. Summary although some acidophilic and alkaliphilic species have been described recently, most of the known sulfate. Sulfatereducing microorganisms srm or sulfatereducing prokaryotes srp are a group composed of sulfatereducing bacteria srb and sulfatereducing archaea sra, both of which can perform anaerobic respiration utilizing sulfate so 4 2 as terminal electron acceptor, reducing it to hydrogen sulfide h 2 s. Ochynski and postgate 123 showed that saltwater strains of desulfovibrio. Several strains of sulfatereducing bacteria were isolated from marine sediments recovered near tunis, korbous and bizerte, tunisia. Characterization of sulfatereducing bacteria isolated from senegal. Samples of subgingival dental plaque were examined for the presence of sulfate.
An alkanedegrading, sulfatereducing bacterial strain, ak01, was isolated from an estuarine sediment with a history of chronic petroleum contamination. Acid mine drainage amd waters are highly acidic ph pdf. Abstract different enrichments are used to isolate sulfate reducers using h 2, lactate, butaryte and ethanol as electron donors. Methods for enrichment and pure culture isolation of filamentous gliding sulfatereducing bacteria. Isolation of a methanogen from deep marine sediments that contain methane hydrates, and description of methanoculleus submarinus sp. Sulfate reducing bacteria srb is a group of microbes that can be used to improve the quality of sediment former coal mine. Isolation and identification of sulfate reducing bacterial. Isolation is done to obtain pure bacterial cultures. Isolation and purification of sulfatereducing bacteria. Considerable simplification in procedure may sometimes be effected by including 3% na2so3.
Vlugt, 1934, sulphatereducing bacteria are physiologically unique among living organisms in. Characterization of sulfatereducing bacteria isolated. Sequence analysis of the 16s rrna gene of sulfatereducing. Sulfatereducing bacteria were isolated from both sulfatecontaining zones. Isolation and characterization of aggregateforming sulfatereducing and purple sulfur bacteria from the chemocline of meromictic lake cadagno, switzerland. Isolation and identification of sulfate reducing bacterial strains. Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment. Characterization of sulfate reducing bacteria isolated.
Note that regrettably, in a previousreport 4, weincorrectly reported a. Quantitative analysis of three hydrogenotrophic microbial groups, methanogenic archaea, sulfatereducing bacteria, and acetogenic bacteria, within plaque biofilms associated with human periodontal disease. In this process, sulfate reduction permits the dissimilatory oxidation of organic matter with the release of. The identification is required so as to cure the illness or the infection caused due to. They have been detected in, and isolated from, many different marine and freshwater. Bioremediation of acid mine drainage using sulfatereducing bacteria 1. Isolation of sulfatereducing bacteria from sediments. Ideal media for clostridia sulfite reducing anaerobes. They all showed characteristics consistent with members of the genus desulfovibrio. In bergeys manual, 5th edition bergey, 1939, they were. In the present study the roll tubes were replaced with serum bottles to cultivate and isolate sulfate reducing bacteria as these are more convenient and reliable. Sulfatereducing bacteria srb represent a class of anaerobic microorganisms that conduct dissimilatory sulfate reduction to obtain energy.
Sulfatereducing bacteria were enumerated in soils and water samples from senegal ricefields using lactate and sulfate as substrates. Sulfur reducing bacteria srb make hydrogen sulfide and have recently been linked as a causative agent in disorders of the gastrointestinal tract, eyes, and respiratory system in humans and animals. Sulphatereducing bacteria have a key role in the sulphur cycle. Pdf isolation of sulfatereducing bacteria from tunisian. Sulfatereducing bacteria an overview sciencedirect topics. This report is the first to describe the occurrence of sulfate.
The ecology and biotechnology of sulphatereducing bacteria. The bacterium is a short, nonmotile, nonsporeforming, gramnegative rod. Sulfatereducing bacteria srb are a widespread group of microorganisms that are often isolated from the anoxygenic environments lake depths, soil, or swamps, and they are also present in the. Pdf isolation and identification of desulfovibrio sp. In this study, sulfate reduction was studied with sediment samples from the extremely acidic tinto river basin. Bioremediation of acid mine drainage using sulfate. The sulfate reducers isolated from the upper 10 m predominantly belonged to the firmicutes figures figures1 1 and and2. Recent advances in the study of the sulfatereducing bacteria ncbi. Abstractthe purpose of this research is to acquire sulfate reducing bacteria srb is used for bioremediation of acid sulfate soils.
This group is often detected in patients with inflammatory bowel disease, including ulcerative colitis. Isolation of sulfatereducing bacteria from tunisian. Methods for enrichment and pure culture isolation of. Cells of strain mb3t were slender, curved, vibrioshaped, motile, gramnegative, nonsporeforming rods. Isolation and characterization of a sulfatereducing. In this study, the occurrence and metabolic capacities of sulfate reducing bacteria srb were studied in 36 water samples taken from cooling towers of 30 different buildings, such as hotels and business centres in istanbul. Crude cultures are readily obtained, but isolation of absolutely pure cultures is usually difficult. Srb were present in 14 cooling towers out of 30 46. Sulfatereducing bacteria, primarily of the genus desulfovibro, are responsible for anaerobic corrosion.
Most sulfatereducing bacteria can also reduce other oxidized inorganic sulfur compounds, such as sulfite, thiosulfateelemental sulfur. Solid and liquid media for isolating and cultivating. The sulfate reducers isolated from the upper 10 m predominantly belonged to the firmicutes figures 1 and 2. The latter shared 97% sequence similarity with its closest described relative desulfosporosinus lacus. Isolation of sulfate reducing bacteria from tunisian marine sediments and description of desulfovibrio bizertensis sp. Purpose this innovative technology report provides an overview of innovative acid mine drainage treatment technologies that employ sulfatereducing bacteria srb. The sulphatereducing bacteria represent the part of the biosphere, the active component in the. Isolation, identification and characterization of bacteria in. Dash et al isolation, characterization and growth control of a sulfate reducing bacteria from sewage water 234 applied ecology and environmental research 103.
1072 1206 939 180 94 1040 1015 1601 666 1634 68 565 1060 120 1562 1054 1315 1281 1484 1149 281 1553 513 948 1135 761 1411 228 910 425 1192 1223 464 824 251 1457 1115 1180 479